The preservation of gonococci in liquid nitrogen
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چکیده
منابع مشابه
The preservation of gonococci in liquid nitrogen.
Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described...
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In recent years some attention has been focused on the low temperature storage of various protozoa. The purpose of this work was to confirm that Trichomonas vaginalis can be preserved for several months at a temperature of -196°C. in liquid nitrogen. Weinman and McAllister (1947), who successfully preserved a number of pathogenic protozoa, failed to preserve T. vaginalis, and only a proportion ...
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There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially pur...
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Liquid nitrogen freezing is recommended for long-term preservation of Leptospira serovars. However, there is no standard protocol to follow for this methodology. We herein report a simple procedure to preserve well-characterized Leptospira serovars unaltered for long-term storage in liquid nitrogen. Forty-three (43) leptospira strains, cryoprotected with 10% (v/v) glycerol were rapidly frozen i...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1971
ISSN: 0021-9746
DOI: 10.1136/jcp.24.2.122